Antioxidant Effects of Pinus eldarica Extracts Against Cisplatin-induced Cytotoxicity in Human Hepatoma (HepG2) Cell Line

Background: Cisplatin has cytotoxic effects through free radical generation. Reports indicate that various members of Pinaceae family may have antioxidant properties. In this study we investigated the cytoprotective needle volatile oil and bark extract Pinus eldarica in Hepatoma G2 (HepG2) cell line. Methods: Using maceration technique, obtained ethanolic eldarica’s (BHAEPE). Folin-ciocalteu reagent was used to determine total phenolic content BHAEPE. The Needle’s Volatile Oil (NVOPE) by Clevenger hydrodistillation method. main components NVOPE were identified Gas Chromatography Mass Spectrophotometry (GC-MS). We DPPH assay investigate activity both Also, MTT performed test protective BHAEPE against cisplatin. Results: demonstrated each gram equivalent 371±6 mg gallic acid. GC-MS germacrene D as component NVOPE. had more capacity compared When incubated solely with cells, neither nor represented cytotoxicity on. Furthermore, mitogenic on higher doses (50, 75 100 μg/ml). However, lower (10, 20 μg/ml) could not protect cells cisplatin’s cytotoxicity, but provided effects. Conclusion: properties did adequately HepG2 cytotoxicity. at high concentrations